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本研究利用RT-PCR技术克隆玉米体细胞胚胎发生关键基因Zm SERK(Gen Bank登录号:KJ0045-22),并构建植物过表达载体p CAMBIA3301-Zm SERK和干扰载体P3301-UBI-Zm SERK(+)-intron-Zm SERK,利用农杆菌介导法将过表达载体、干扰载体、空载体转入供试品种的玉米体细胞胚胎中,得到阳性植株,阳性率分别为11.5%、12.5%、9.1%。随后对阳性植株进行分子检测和表达分析。
In this study, we cloned the Zm SERK (GenBank accession number: KJ0045-22), a plant overexpression vector p CAMBIA3301-Zm SERK and the interference vector P3301-UBI-Zm SERK (+ ) -intron-Zm SERK. The over-expression vector, the interference vector and the empty vector were transferred into maize somatic embryos by Agrobacterium-mediated method. The positive rates were 11.5%, 12.5%, 9.1 %. Positive plants were then subjected to molecular detection and expression analysis.