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[目的]从现有的草莓ESTs数据中获取有效的微卫星标记。[方法]利用MISA(Microsatellite)软件分析简单重复序列(simple sequence repeats,SSRs)在草莓EST中的分布频率与密度,用CAP3软件进行冗余分析。[结果]在17565条EST序列中,共获得10129条SSR序列,SSRs之间的距离约为0.90kb。其中,六碱基重复丰度最大,占61.0%,而三碱基、单碱基、二碱基、四碱基和五碱基重复丰度分别为14.3%,13.1%,6.2%,4.3%和1.1%。在单碱基、二碱基、三碱基和四碱基重复模体中,丰度最大的分别是A/T,AG,AAG和AAAG,而CG在编码区内没有。在这6种类型的重复模体中,冗余与非冗余的草莓EST之间没有显著差异。[结论]从现有的草莓ESTs数据中可以方便地获取有效的微卫星标记。
[Objective] To obtain effective microsatellite markers from the existing strawberry ESTs data. [Method] The frequency and density of simple sequence repeats (SSRs) in strawberry ESTs were analyzed by MISA (Microsatellite) software, and the redundancy was analyzed by CAP3 software. [Result] A total of 10129 SSR sequences were obtained from 17565 ESTs, the distance between SSRs was about 0.90kb. Among them, the six-base repeat abundance was the largest, accounting for 61.0%, while the repeated abundances of three bases, one base, two bases, four bases and five bases were 14.3%, 13.1%, 6.2% and 4.3% And 1.1%. Among single-, two-, three- and four-base repeat motifs, the most abundant were A / T, AG, AAG and AAAG, respectively, while CG was absent in the coding region. There was no significant difference between redundant and non-redundant strawberry ESTs among these six types of repeat motifs. [Conclusion] The effective microsatellite markers could be conveniently obtained from the existing ESTs data of strawberry.