论文部分内容阅读
目的检测受大剂量X射线照射的大鼠晶状体中可溶性αB-晶体蛋白的表达水平,探讨αB-晶体蛋白与放射性白内障形成之间的关系。方法取8周龄SD大鼠24只,随机分为3组,每组8只。正常对照组未予干预措施;实验照射组采用直线加速器(X射线25 Gy)一次性照射SD大鼠双眼,建立放射性白内障模型;实验对照组予假照射(照射源不含射线,余条件同照射组)。照射后3个月处死大鼠,取晶状体作匀浆,取其上清(可溶性晶状体蛋白)用Western blot检测αB-晶体蛋白的表达。结果正常对照组、实验对照组均未出现晶状体混浊,25 Gy照射组逐渐形成典型放射性白内障;Western blot结果显示,照射组SD大鼠眼晶状体内可溶性αB-晶体蛋白含量较实验对照组明显降低,差异有统计学意义(P<0.01)。结论X射线可降低晶状体中可溶性αB-晶体蛋白的含量,从而导致白内障。
Objective To detect the expression of soluble αB-crystallin in rat lens exposed to large doses of X-rays and to explore the relationship between αB-crystallin and radiation-induced cataract formation. Methods Twenty-four SD rats aged 8 weeks were randomly divided into 3 groups with 8 rats in each group. The normal control group was given no intervention. The experimental group was irradiated with 25 Gy X-ray and the both eyes of SD rats were irradiated with one time irradiation to establish the model of radiation cataract. The experimental group was given sham irradiation group). The rats were sacrificed 3 months after irradiation and the lens was homogenized. The supernatant (soluble lens protein) was taken for Western blot to detect the expression of αB-crystal protein. Results No lens opacity was found in normal control group and experimental control group, and typical radiation cataract was gradually formed in 25 Gy irradiation group. Western blot results showed that the content of soluble α B-crystallin in lens group of SD rats in irradiation group was significantly lower than that in experimental control group, The difference was statistically significant (P <0.01). Conclusion X-rays can reduce the content of soluble αB-crystallin in the lens and lead to cataract.