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目的 :观察大鼠肝胆代谢功能及雌激素对胆汁分泌的反应。方法 :将 2 0只近交系Wistar大鼠随机分组 ,试验组肌肉注射苯甲酸雌二醇 ,由丙三醇稀释 ,5mg/kg体重 ,连续注射 5d。对照组肌肉注射丙三醇。所有动物于第 6天手术装置急性胆总管瘘管 ,连续收集 4次胆汁 ,前 3次每隔 2 0min收集 1次 ,第 4次收集 1h。于胆汁收集前后采血 ,测定血浆与胆汁中总胆酸 (TBA)、甘氨胆酸 (GC)、总胆红素 (TBIL)、丙氨酸转氨酶 (ALT)、天冬酸转氨酶 (AST)、碱性磷酸酶 (ALKP)、γ 谷氨酰转肽酶 (GGT)、总胆固醇(CHO)等肝胆功能指标及激素水平 ,作统计分析。结果 :大鼠胆汁中TBA、GC、TBIL、GGT显著高于血浆中浓度 ,而血浆中ALT、AST、ALKPCHOL显著高于胆汁中浓度。胆汁中TBA、GC极高 ,而CHOL、ALT、AST、ALKP、雌二醇 (E2 )甚微。雌激素注射可使胆汁分泌量显著减少 ,胆流速度显著减慢。雌激素注射后 ,血浆中CHOL显著下降 ,ALKP、GGT、E2 显著升高 ,胆汁中TBA显著下降 ,TBIL、ALKP、GGT、CHO显著升高 ,其它指标变化不大。结论 :雌激素可降低胆汁分泌功能 ,引起肝内淤胆 ,致血浆和胆汁中GGT、ALKP、E2 的升高 ,但不直接损伤肝细胞。雌激素可导致CHOL肝内合成减少而胆内排泄加快 ,但不能引起血液和胆汁中胆酸浓度发生变化。
Objective: To observe the metabolism of liver and gallbladder in rats and the response of estrogen to bile secretion. Methods: Twenty inbred Wistar rats were randomly divided into experimental group, estradiol benzoate intramuscular injection, diluted with glycerol, 5mg / kg body weight, continuous injection 5d. Control group intramuscular injection of glycerol. All animals were operated on acute choledocholithotomy tube on the 6th day. The bile was collected 4 times in succession. The first three times were collected every 20 minutes and the fourth time was collected for 1 hour. Blood samples were taken before and after bile collection, and the levels of total bile acid (TBA), glycocholic acid (GC), total bilirubin (TBIL), alanine aminotransferase (ALT), aspartate aminotransferase (AST) Alkaline phosphatase (ALKP), γ-glutamyl transpeptidase (GGT), total cholesterol (CHO) and other indicators of liver and gallbladder and hormone levels for statistical analysis. Results: The levels of TBA, GC, TBIL and GGT in rat bile were significantly higher than those in plasma while ALT, AST and ALKPCHOL in plasma were significantly higher than those in bile. Bile TBA, GC high, and CHOL, ALT, AST, ALKP, estradiol (E2) is very small. Estrogen injection can significantly reduce the secretion of bile, biliary flow significantly slowed down. After injection of estrogen, CHOL in plasma was significantly decreased, ALKP, GGT and E2 were significantly increased, TBA in bile was significantly decreased, TBIL, ALKP, GGT and CHO were significantly increased, while other indexes did not change much. Conclusion: Estrogen can reduce the secretion of bile, causing intrahepatic cholestasis, resulting in the increase of GGT, ALKP and E2 in plasma and bile, but not direct damage of hepatocytes. Estrogen can lead to reduced CHOL intrahepatic synthesis and excretion of bile faster, but can not cause blood and bile cholic acid concentration changes.