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比较T-2毒素对不同生长期凡纳滨对虾(Litopenaeus vannamei)的急性毒性及其分析方法,明确对虾作为T-2毒素毒性评价的生物标记物的最佳时期,建立T-2毒素对对虾的急性毒性互补评价方法。通过对不同生长期凡纳滨对虾的急性毒性试验,采用寇氏法和概率单位法,计算得到T-2毒素对不同生长期凡纳滨对虾的LD_(50),并通过u检验对两种方法求得的LD_(50)值进行分析比较。结果得到T-2毒素对虾卵、幼虾和成虾的LD_(50)值分别为2.33、1.79和3.34 mg/kg·bw,且经检验两种方法求得的LD_(50)并无显著性差异。研究表明,T-2毒素对成虾的毒害作用最小急性毒性最弱,对幼虾的毒害作用最大急性毒性最强,即该生长期的凡纳滨对虾更适宜做评价T-2毒素急性毒性的生物标志物,同时采用寇氏法和概率单位法相结合的方法得到的LD_(50)值更具准确。
To compare the acute toxicity of T-2 to Litopenaeus vannamei at different growth stages and its analytical method, the best period of shrimp as a biomarker for T-2 toxin toxicity was established. T-2 toxin Acute toxicity complementary evaluation method. Based on the acute toxicity test of Litopenaeus vannamei at different growth stages, the LD 50 of T-2 toxin was determined by Kovar’s method and probability unit method. The LD_ (50) Method to obtain the value of LD_ (50) for analysis and comparison. Results The LD_ (50) values of T-2 toxin, shrimp and shrimp were 2.33, 1.79 and 3.34 mg / kg · bw, respectively. There was no significant difference between the two methods difference. The results showed that the T-2 toxin had the least acute toxicity to the adult shrimp and the strongest toxic to the juvenile shrimp, namely, the juvenile P. vannamei was more suitable to evaluate the acute toxicity of T-2 toxin Of the biomarkers, while the use of Kor’s method and the combination of probability units LD_ (50) value is more accurate.