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目的探讨对氨基水杨酸钠(PAS-Na)对染锰大鼠血清脂质过氧化、血和骨骼金属元素水平的影响。方法28只SD雄性大鼠被随机分为对照组、染锰组、低、高剂量PAS-Na治疗(L-、H-PAS)组。染锰组、L、H-PAS组腹腔注射(ip)Mn Cl2·4H2O 15 mg/kg,对照组ip等容量生理盐水,每日1次,每周5 d,连续3周。然后,L、H-PAS组背部皮下注射(sc)PAS-Na 100和200 mg/kg,对照组和染锰组背部sc等容量生理盐水,每日1次,连续5周。测定血清超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活性、丙二醛(MDA)含量,测定血、股骨、髂骨锰(Mn)、铁(Fe)、锌(Zn)、铜(Cu)、钙(Ca)和镁(Mg)含量。结果与对照组比较,染锰组血清MDA含量增高,H-PAS组MDA含量降低(P<0.05)。血、股骨、髂骨Mn含量比对照组高,L-PAS组比对照组低(P<0.05);染锰组血Fe、Mg含量均高于对照组,Zn含量低于对照组,L-PAS组Mg含量降低(P<0.05);染锰组髂骨Cu、Mg含量均低于对照组(P<0.05)。结论过量锰暴露可致机体产生脂质过氧化,干扰Fe、Cu、Mg、Zn含量的分布。PAS-Na有促排锰作用,对锰致血脂质过氧化和镁含量影响有干预作用。
Objective To investigate the effect of sodium salicylate (PAS-Na) on serum lipid peroxidation, blood and skeletal metal elements in rats with manganese exposure. Methods Twenty-eight SD male rats were randomly divided into control group, manganese group, low and high dose PAS-Na treatment group (L-, H-PAS). The rats in the manganese-treated group and the rats in the L and H-PAS groups were intraperitoneally injected with Mn Cl2.4H2O (15 mg / kg) and the control group (ip) with the same volume of normal saline once a day for 5 days a week for 3 weeks. Then, PAS-Na 100 and 200 mg / kg were injected subcutaneously into the back of the L, H-PAS group, sc and normal saline in the control group and back of the manganese-dosed group once a day for 5 weeks. Serum levels of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and malondialdehyde (MDA) , Zinc (Zn), copper (Cu), calcium (Ca) and magnesium (Mg) content. Results Compared with the control group, serum MDA level in Mn-Mn group increased and MDA content in H-PAS group decreased (P <0.05). The content of Mn in blood, femur and ilium was higher than that in control group, while L-PAS group was lower than that in control group (P <0.05). The levels of Fe and Mg in blood were higher than those in control group, The content of Mg in PAS group decreased (P <0.05). The content of Cu and Mg in ilium bone in Mn-Zn group was lower than that in control group (P <0.05). Conclusion Excess manganese exposure may cause lipid peroxidation in the body and interfere with the distribution of Fe, Cu, Mg and Zn. PAS-Na has a role in promoting the discharge of manganese, manganese-induced lipid peroxidation and magnesium content intervention effect.