材料与方法一、动物采用雄性大白鼠,体重200—250克,共30只,分为6组,每组5只。染毒前由每组抽样一只断颈活杀,编为一号动物作为空白组;二号动物为配对对照组。各组剩下的4只,将其持续染毒4小时,活杀。取肺组织迅速放入10%甲醛溶液中,固定3—5天,石蜡包埋,苏木精、嗜伊红染色、制片、镜检。二、染毒装置与方法染毒装置用容积为41升的玻璃罩,罩上 Materials and methods First, the animals used male rats, weighing 200-250 grams, a total of 30, divided into 6 groups of 5. Before exposure, one sample was taken from each group to kill the neck, and numbered as animal one as a blank group; animal number two as a matched control group. The remaining four in each group, the continued exposure to poison for 4 hours, kill live. Take lung tissue quickly into 10% formaldehyde solution, fixed 3-5 days, embedded in paraffin, hematoxylin, eosin staining, filming, microscopic examination. Second, the device and method of exposure The device with a volume of 41 liters glass cover, cover