研究了甲醇、乙醇、丙醇（正丙醇和异丙醇）、丁醇（正丁醇、异丁醇、仲丁醇和叔丁醇）、乙二醇、丙二醇（正丙二醇和异丙二醇）和丙三醇（甘油）的细胞毒性，分析了甲醇、乙醇、丙醇和丁醇诱导细胞表达ＨＳＰ６８的浓度与其细胞毒性的关系，发现醇的结构和细胞毒性有密切关系，一元醇的烃链越长，细胞毒性越大，多元醇的烃基数与羟基数的比值越大，细胞毒性越大；一元醇的正某醇细胞毒性大于相应的异某醇。醇诱导细胞细胞表达ＨＳＰ６８的浓度低于相应醇的ＬＤ５０值的１４％（ＬＤ３５±６）。 Methanol, ethanol, propanol (n-propanol and isopropanol), butanol (n-butanol, isobutanol, sec-butanol and tert-butanol), ethylene glycol, propylene glycol The results showed that the structure and cytotoxicity of alcohol were closely related. The longer the hydrocarbon chain of monohydric alcohol, The greater the cytotoxicity, the greater the ratio of the number of hydroxyl groups to the number of hydroxyl groups of the polyol, the greater the cytotoxicity; the positive alcohol toxicity of the monohydric alcohol is greater than that of the corresponding isomalcohol. Alcohol-induced cell-cell expression of HSP68 was less than 14% (LD35 ± 6) of the LD50 of the corresponding alcohol.