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目的建立一种可用于早产儿氨茶碱血液浓度的检测方法。方法以氨茶碱为模板分子,吡咯为功能单体,在0.2 mol/L的HAc-Na Ac缓冲液(p H 4.0)中,通过电聚合方法在玻碳电极表面聚合形成氨茶碱分子印迹传感膜;利用三维激光扫描显微镜、差分脉冲伏安法(DPV)和电化学交流阻抗法(EIS)对分子印迹传感膜的表面形貌及性能进行表征;在5 mmol/L K3[Fe(CN)6]-0.1 mol/L KCl溶液中,采用方波伏安法(SWV)考察了分子印迹膜的聚合扫描圈数和孵育时间对传感器响应的影响。结果在优化实验参数下,传感器SWV峰电流差值与氨茶碱的浓度负对数在1×10-7~1×10-3mol/L范围呈现良好的线性关系,检出限为0.5×10-8mol/L(S/N=3),加标回收率为92.2%~101.4%;构建的氨茶碱分子印迹电化学传感器具有良好的选择性、稳定性和重现性。结论所构建的氨茶碱分子印迹电化学传感器有望用于临床上早产儿血液氨茶碱分子浓度的快速及准确检测。
Objective To establish a method for the determination of aminophylline blood concentration in preterm infants. Methods Using aminophylline as the template and pyrrole as the functional monomer, aminophylline was molecularly imprinted on the surface of glassy carbon electrode by electropolymerization in 0.2 mol / L HAc-Na Ac buffer (p H 4.0) The surface morphology and properties of the molecularly imprinted sensing films were characterized by three-dimensional laser scanning microscopy, differential pulse voltammetry (DPV) and electrochemical impedance spectroscopy (EIS). In the presence of 5 mmol / L K3 [Fe (CN) 6] -0.1 mol / L KCl solution, the influence of the number of scanning cycles and the incubation time on the sensor response was investigated by using square wave voltammetry (SWV). Results Under the optimal experimental parameters, the difference between the SWV peak current and the negative logarithm of aminophylline showed a good linearity in the range of 1 × 10-7 ~ 1 × 10-3 mol / L with a detection limit of 0.5 × 10 -8mol / L (S / N = 3). The recoveries were 92.2% -101.4%. The constructed aminophylline molecularly imprinted electrochemical sensor has good selectivity, stability and reproducibility. Conclusion The constructed aminophylline molecularly imprinted electrochemical sensor is expected to be used for the rapid and accurate detection of the concentration of aminophylline in the blood of clinically premature infants.