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目的探讨益气活血解毒中药对胶原诱导性关节炎(CIA)大鼠炎症细胞因子及NF-κB信号通路关键因子表达的影响。方法以牛Ⅱ型胶原尾根及背部多点注射制作CIA大鼠模型,以免疫抑制剂来氟米特为阳性对照药物,益气活血解毒中药干预,病理切片HE染色,观察大鼠足跖关节的病理改变,细胞因子芯片检测CIA大鼠血清中炎症细胞因子的含量,real-time PCR检测NF-κB信号通路中关键因子的基因表达改变。结果益气活血解毒中药可以明显抑制关节滑膜组织增生,缓解炎性渗出,缩小纤维素样坏死区范围,并显著降低CIA大鼠外周血血清中肿瘤坏死因子(TNF)α、白细胞介素(IL)-1β、IL-5、IL-6、IL-10的含量,NF-κB mRNA表达与模型组相比均显著下降(P<0.01或P<0.05),NF-k B抑制蛋白激酶mRNA表达有下降趋势,统计学分析差异无显著性。结论益气活血解毒中药可以通过干预与类风湿关节炎(RA)相关的炎症细胞因子和NF-κB信号通路相关因子的表达,抑制病变组织的炎症反应,修复炎症所导致的关节损伤,达到治疗RA的目的,这是益气活血解毒中药干预RA免疫炎症反应的关键作用机制之一。
Objective To investigate the effect of Yiqi Huoxue Jiedu Chinese medicine on the expression of key cytokines and NF-κB signaling in collagen-induced arthritis (CIA) rats. Methods CIA rat model was made by injection of bovine type Ⅱ collagen into the tail and back of the rats. The immunosuppressive leflunomide was used as a positive control drug, Yiqi Huoxue Huoxue Jiedu Chinese medicine intervention and pathological section HE staining. The changes of inflammatory cytokines in serum of CIA rats were detected by cytokine chip. The gene expression of key factors in NF-κB signaling pathway was detected by real-time PCR. Results Yiqi Huoxue Huoxie Jiedu Decoction could significantly inhibit the proliferation of synovial tissue, relieve inflammatory exudation, narrow the range of fibrinoid necrosis, and significantly reduce the serum levels of tumor necrosis factor (TNF) α, interleukin The levels of IL-1β, IL-5, IL-6 and IL-10 in the model group were significantly lower than those in the untreated group (P <0.01 or P < mRNA expression decreased, no significant statistical difference. Conclusion Yiqi Huoxue Jiedu can inhibit the inflammatory reaction of diseased tissues and repair the joint damage caused by inflammation by interfering with the expression of inflammatory cytokines and NF-κB signaling pathways associated with rheumatoid arthritis RA purposes, which is one of the key mechanism of Yiqi Huoxue Jiedu Chinese medicine intervention in RA immune inflammation.