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目的:研究黄芩苷对光滑念珠菌、近平滑念珠菌、克柔念珠菌、季也蒙念珠菌等非白念珠菌生物膜的影响。方法:96孔微量培养板上构建4种非白念珠菌生物膜;微量稀释法检测黄芩苷对4种非白念珠菌最低抑菌浓度(MIC);XTT减低法评价黄芩苷对4种非白念珠菌生物膜SMIC及菌细胞黏附性的影响。结果:4种非白念珠菌在96孔板中可形成成熟的生物膜;黄芩苷对4种非白念珠菌MIC分别是125,250,125,62.5 mg.L-1;黄芩苷对4种非白念珠菌生物膜的SMIC50分别是(1 000,500,125,250 mg.L-1;SMIC80均大于或等于1 000 mg.L-1。黄芩苷对4种非白念珠菌的黏附有一定的抑制效应。结论:黄芩苷对近平滑念珠菌、克柔念珠菌、季也蒙念珠菌生物膜有较强的抑制作用。
Objective: To study the effects of baicalin on Candida glabrata, Candida parapsilosis, Candida krusei, Candida albicans and other non-Candida albicans biofilms. Methods: Four non-Candida albicans biofilms were constructed on 96-well microtiter plates. The minimum inhibitory concentrations (MICs) of baicalin against four non-Candida albicans strains were determined by micro-dilution method. Candida albicans SMIC and bacterial cell adhesion. Results: Four kinds of non-Candida albicans could form mature biofilm in 96-well plates. The MICs of baicalin to four kinds of non-Candida albicans were 125, 250, 125 and 62.5 mg.L-1, respectively. The biofilm SMIC50 were (1 000, 500, 125 and 250 mg.L-1, respectively); SMIC80 was greater than or equal to 1 000 mg.L-1. Baicalin had some inhibitory effect on the adhesion of four non-Candida albicans.Conclusion: Candida parapsilosis, Candida krusei, season also Candida biofilm has a strong inhibitory effect.