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为了优化红掌的离体再生条件,本研究以红掌“粉冠军”无菌苗的根尖为外植体,对愈伤诱导、不定芽再生及植株生根过程中植物生长调节剂的浓度进行优化,同时通过半薄切片技术对愈伤发生和分化过程进行组织学观察。试验结果表明,“粉冠军”根尖置于培养基1/2 MS,添加0.2 mg/L噻苯隆(TDZ)和30 g/L蔗糖,黑暗下培养2个月后,外植体出愈率达到86%。将愈伤组织转接到MS基本培养基,同时添加1.0 mg/L吡效隆(CPPU)和25 g/L蔗糖,16 h/8 h光照周期条件下不定芽的分化率达到100%,发生数量平均为6.6个/块。将不定芽转接于添加0.5 mg/L吲哚丁酸(IBA)、0.4 g/L活性炭和25 g/L蔗糖的MS培养基上,根系发育良好。组织切片观察结果表明,愈伤起源于根尖分生组织,而不定芽起源于愈伤表面组织。研究建立了红掌“粉冠军”的一种离体再生方法,并为红掌愈伤组织的形态发生研究提供参考。
In order to optimize the regeneration conditions of Anthurium andraeanum, the root tip of the seedlings of Anthurium androphis was used as explants. The effects of callus induction, regeneration of adventitious buds and plant growth regulators Concentration was optimized, and at the same time by semi-thin section of the callus and differentiation process of histological observation. The results showed that the root tips of “Pink Champion ” were placed in 1/2 MS medium and 0.2 mg / L TDZ and 30 g / L sucrose were added to the dark for 2 months. Out of 86%. The callus was transferred to MS basal medium with addition of 1.0 mg / L Piperonylpolypyridin (CPPU) and 25 g / L sucrose, and the differentiation rate of adventitious buds reached 100% under 16 h / 8 h photoperiod The average number of 6.6 / block. Adventitious buds were transferred to MS medium supplemented with 0.5 mg / L indole butyric acid (IBA), 0.4 g / L activated charcoal and 25 g / L sucrose. Roots developed well. Tissue section observation showed that the callus originated in the apical meristem, and adventitious buds originated in the callus surface tissue. The study established an in vitro regeneration method of Anthurium and “Pink Champion” and provided reference for the morphogenesis of Anthurium andraeanum.