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目的 :探讨髓系白血病患者外周血T淋巴细胞核仁形成区嗜银蛋白 (Ag -NORs)测定的临床意义。方法 :采用北京健尔康医疗设备有限公司提供的KL型肿瘤免疫图像分析系统及配套试剂对 42例慢性粒细胞白血病及非淋巴细胞白血病的血液恶性肿瘤病人外周血T淋巴细胞进行染色体分析。结果 :慢性粒细胞白血病患者T淋巴细胞Ag -NORs的核仁银染面积与核面积比值 (1.5 % )为 1.77± 7.95 ,急性非淋巴细胞白血病患者 (1.5 % )为 2 .5 7± 7.3 7与正常对照组 (1.5 % ) 6.14± 9.45比较 0 .0 0 0 =P <0 .0 1。结论 :T淋巴细胞核仁形成区嗜银蛋白检测不仅可准确反映髓系白血病患者的免疫状态 ,而且与髓系白血病的诊断 ,鉴别诊断 ,病情发展呈良好的相关性 ,同时高度的特异性 ,灵敏性及检测的简便 ,可作为判断髓系白血病的转归的临床追踪观察指标值得进一步研究。
Objective: To investigate the clinical significance of the determination of Ag-NORs in the peripheral blood T lymphocytes of myeloid leukemia patients. Methods: Chromosome analysis of peripheral blood T lymphocytes from 42 patients with chronic myelogenous leukemia and non-lymphocytic leukemia was performed by using KL tumor immunoassay system and its accessory reagent provided by Beijing Jian’erkang Medical Equipment Co., Ltd. Results: The ratios of nucleolar silver to nucleus area (1.5%) of T-lymphocyte Ag-NORs in patients with chronic myeloid leukemia were 1.77 ± 7.95 and those in acute non-lymphocytic leukemia patients (1.5%) were 2.57 ± 7.37 Compared with the normal control group (1.5%) 6.14 ± 9.45 0 .0 0 0 = P <0. CONCLUSION: The detection of argyrophilic protein in nucleolar organizer of T lymphocytes not only accurately reflects the immune status of patients with myeloid leukemia, but also has a good correlation with the diagnosis, differential diagnosis and progression of myeloid leukemia, and is highly specific and sensitive Sex and detection of simple, can be used as a prognosis of myeloid leukemia clinical follow-up indicators worth further study.