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目的探讨大鼠海马区在全脑缺血及再灌注状态下NO浓度变化的动力学。方法通过四动脉结扎造成大鼠全脑缺血模型,应用NO电极对大鼠海马区在全脑缺血及再灌注状态下NO浓度变化进行实时检测。结果按照时间进程和NO浓度的变化量,整个NO浓度变化过程可以分为5个时相:第一时相,在缺血早期,有一个NO浓度的显著下降,从开始下降到下降到最低点,在时间上大约为5min;第二时相,NO下降开始到最低点后,出现一个NO浓度变化的稳定期,时间上约占4min;第三时相,轻度的NO浓度上升,直到再灌开始,该时相大约为10min;第四时相,一旦再灌开始,有一个急性上升期,维持时间为4min;第五时相,为另一稳定期,约为1h。结论在全脑缺血过程中,海马区NO浓度的变化具有波动性,导致NO浓度波动变化的机理有待于进一步研究。
Objective To investigate the kinetics of NO concentration in rat hippocampus during global cerebral ischemia and reperfusion. Methods The rat model of global cerebral ischemia was established by ligation of four arteries. NO concentration was measured in hippocampus of rat hippocampus after global ischemia and reperfusion. Results According to the time course and the change of NO concentration, the change of NO concentration could be divided into five phases: the first phase, there was a significant decrease of NO concentration in the early stage of ischemia from the beginning to the lowest point , The time is about 5min; the second phase, NO decreased to the lowest point began to appear after a stable concentration of NO changes in time about 4min; the third phase, mild NO concentration increased until The beginning of irrigation, the time phase is about 10min; the fourth phase, once the reperfusion began, there is an acute period, the maintenance time is 4min; fifth phase, for another stable period of about 1h. Conclusion During the process of global cerebral ischemia, the concentration of NO in hippocampus fluctuates. The mechanism of fluctuating NO concentration remains to be further studied.