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[目的]探讨幽门螺杆菌(Hp)感染时胃黏膜组织一氧化氮(NO)水平及其与细胞凋亡的关系。[方法]应用快速脲酶法及PCR法对132例胃病患者胃黏膜进行Hp检测,其中Hp阳性75例,Hp阴性57例;病理学分型采用悉尼分型法;用亚硝基还原法检测血清中NO水平及荧光染色法检测胃黏膜细胞的凋亡情况。[结果]Hp阳性组血清NO水平高于Hp阴性组(P<0.01),在Hp阳性患者中,血清NO水平胃癌(GCA)>萎缩性胃类(CAG)>浅表性胃炎(CSG)(P<0.01)。凋亡指数(AI)的比较:Hp阳性组高于Hp阴性组(P<0.01),CAG>CSG(P<0.01),GCA与CSG之间比较差异无统计学意义。[结论]Hp感染与胃黏膜的萎缩、肠化生等癌前病变的关系密切;Hp诱导产生的NO与GCA的形成发展密切相关。对NO水平的调控可望成为治疗癌前病变,阻止GCA形成的有效措施。
[Objective] To investigate the relationship between the level of nitric oxide (NO) in gastric mucosa and the apoptosis of Helicobacter pylori (Hp) infection. [Method] The gastric mucosa of 132 gastric patients were detected by rapid urease method and PCR method. Among them, 75 were positive for Hp and 57 were negative for Hp. The pathological typing was performed by Sydney typing method and nitrosoguanidine The levels of NO and fluorescence staining were used to detect the apoptosis of gastric mucosal cells. [Results] The serum NO level in Hp positive group was higher than that in Hp negative group (P <0.01). In Hp positive patients, serum NO levels were significantly higher in GCA> CAG> CSG ( P <0.01). Apoptotic index (AI): Hp positive group was higher than Hp negative group (P <0.01), CAG> CSG (P <0.01), GCA and CSG was no significant difference between the statistically significant. [Conclusion] Hp infection is closely related to gastric mucosal atrophy, intestinal metaplasia and other precancerous lesions. NO produced by Hp is closely related to the formation and development of GCA. The regulation of NO level is expected to become an effective measure to prevent the formation of precancerous lesions and prevent the formation of GCA.