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采用本实验室自行制备的反相高效液相色谱柱，以醋酸钠缓冲液和乙腈－水线性梯度洗脱，在室温下对常见的１６种１－氟－２， ４－二硝基苯（ＦＤＮＢ）衍生化氨基酸进行了有效的分离，用紫外吸收检测器（３６０ｎｍ）和外标定量法对灵芝蛋白中各种氨基酸的含量进行了测定。结果表明：ＦＤＮＢ衍生化氨基酸在一周内是稳定的，回收率在９６％～１０２％之间，相对标准偏差小于４％，灵芝中含有人体必需的赖氨酸、苯丙氨酸和组氨酸等组分。
Using a self-prepared reversed-phase HPLC column in the laboratory, eluted with a linear gradient of sodium acetate buffer and acetonitrile-water, the common 16 kinds of 1-fluoro-2,4-dinitrobenzene (at room temperature) The FDNB) derivatized amino acids were effectively separated, and the contents of various amino acids in the ganoderma lucidum protein were determined using an ultraviolet absorption detector (360 nm) and an external standard quantification method. The results showed that the FDNB derivatized amino acids were stable in one week, the recovery rate was between 96% and 102%, and the relative standard deviation was less than 4%. Ganoderma lucidum contains essential lysine, phenylalanine and histidine. Other components.