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牙釉骨质界的形态研究差异较大,其原因为电镜或光镜切片制备中的方法问题。本研究用牙釉骨质界复制品和原标本磨片,分别用扫描电镜(SEM)和偏光显微镜研究了牙釉骨质界的结构形态。 用正畸拔除的而不损伤牙釉骨质界的50颗年轻前磨牙,置于缓冲的10％福尔马林中。研究前浸入5％次氯酸钠溶液中20分钟,然后冲洗5分钟,去除软组织,分别标记颊舌侧,切去部分冠根制成含适量冠方釉质和
The morphological study of dental enamel bone boundaries vary greatly, due to the methodological issues in the preparation of electron microscopy or light microscopy. In this study, the morphological changes of dental enamel boundary were studied by means of scanning electron microscopy (SEM) and polarized light microscopy (TEM). Fifty young premolars extracted with orthodontics without damaging the dental enamel bone boundaries were placed in buffered 10% formalin. Before the study immersed in 5% sodium hypochlorite solution for 20 minutes and then rinsed for 5 minutes to remove soft tissue, respectively, mark the buccal and lingual side, cut off part of the crown to make with the amount of crown enamel and