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目的:研究化痔栓中人参皂苷Rg1、三七皂苷R1的含量测定方法。方法:采用高效液相色谱法进行梯度洗脱,色谱柱为C18柱(4.6mm×250mm,5μm),流动相为乙腈∶水(19∶81),乙腈∶水(36∶64),流速为1.0mL/min,检测波长为203nm,柱温为室温。结果:人参皂苷Rg1、三七皂苷R1的线性良好(r=0.9998、r=0.9997),线性范围人参皂苷Rg197.6～976.0μg/mL,三七皂苷R122.0～220.0μg/mL。人参皂苷Rg1、三七皂苷R1的回收率分别为97.82%、97.60%;RSD值分别为1.26%、1.84%,n=5。结论:该方法简便,灵敏,重现性好,可作为化痔栓中人参皂苷Rg1、三七皂苷R1的含量测定方法。
Objective: To study the method of determination of ginsenoside Rg1 and notoginsenoside R1 in Huishishe suppository. Methods: The gradient elution was performed on a C18 column (4.6 mm × 250 mm, 5 μm) using acetonitrile as the mobile phase (19:81), acetonitrile: water (36:64) at a flow rate of 1.0mL / min, detection wavelength of 203nm, the column temperature is room temperature. Results: Ginsenoside Rg1, notoginsenoside R1 had good linearity (r = 0.9998, r = 0.9997), linear range ginsenoside Rg197.6 ~ 976.0μg / mL and notoginsenoside R122.0 ~ 220.0μg / mL. The recoveries of ginsenoside Rg1 and notoginsenoside R1 were 97.82% and 97.60%, respectively. The RSDs were 1.26% and 1.84%, respectively, and n = 5. Conclusion: The method is simple, sensitive and reproducible. It can be used as a method for determination of ginsenoside Rg1 and notoginsenoside R1 in Huishishe suppository.