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目的 研究人乳头状瘤病毒 (HPV)与促癌物协同作用在scid小鼠体内诱发人胚宫颈细胞的恶性转化。方法 包装制备含HPV1 6E6E7逆转录病毒 ,感染人胚宫颈细胞 ,按分组将所需细胞移植于scid小鼠右侧肩部皮下 ,共分 4组 :实验组为感染病毒的宫颈细胞 +亚精胺 +正丁酸组 ,共 7只 ;病毒组为感染病毒的宫颈细胞组 ,共 5只 ;促癌组为正常宫颈细胞 +亚精胺 +正丁酸组 ,共 5只 ;对照组为正常宫颈细胞组 ,共 4只。按实验要求于移植第 3日起在小鼠左侧肩部皮下注射亚精胺和正丁酸 ,每周 1次。观察 1 2周后处死动物 ,如发生肿瘤 ,则对瘤组织行病理诊断 ,并作PCR检测HPV1 6E6E7基因。结果 实验组成瘤比例为 5/ 7,其他 3组成瘤率皆为 0 ,实验组肿瘤的病理学检查证实为肉瘤 ,应用PCR方法在肿瘤组织中检测到HPV1 6E6E7基因。结论 宫颈细胞在感染含HPV1 6E6E7基因的逆转录病毒后 ,在亚精胺和正丁酸协同作用下发生恶性转化
Objective To study the synergistic effect of human papilloma virus (HPV) and carcinogens in the induction of malignant transformation of human cervical epithelial cells in scid mice. Methods HPV16E6E7 retrovirus was infected and infected into human embryo cervical cells. The desired cells were transplanted into the right shoulder of scid mice subcutaneously in 4 groups. The experimental group was infected with virus-infected cervical cells + spermidine + N-butyric acid group, a total of 7; the virus group is a group of cervical cells infected with virus, a total of 5; cancer-promoting group of normal cervical cells + spermidine + n-butyric acid group, a total of 5; Cell group, a total of 4. According to the experimental requirements on the 3rd day after transplantation in mice left shoulder subcutaneous injection of spermidine and n-butyric acid, once a week. The animals were sacrificed after 12 weeks of observation. In the case of tumors, pathological diagnosis of the tumor tissues was performed and HPV16E6E7 gene was detected by PCR. Results The rate of tumor formation in the experimental group was 5/7, and that in the other 3 groups was all 0. The pathological examination of the tumor in the experimental group proved to be sarcoma. HPV1 6E6E7 gene was detected in the tumor tissue by PCR. Conclusion Cervical cells undergo malignant transformation under the synergic effect of spermidine and n-butyric acid after infection with retrovirus containing HPV1 6E6E7 gene