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目的观察环磷酰胺对脑缺血再灌注后大鼠大脑海马区胶原纤维酸性蛋白(GFAP)表达的影响。方法设正常组、假手术组、缺血再灌注组(I/R)和环磷酰胺处理组(T),以改良Longa线栓法建立SD大鼠局灶性脑缺血模型。处理组缺血后1h给予环磷酰胺腹腔注射,处理组和I/R组均于缺血后2h形成再灌注,每组均分为三个时间段(24h、72h和5d)分别观察。应用免疫组织化学技术检测各组脑组织不同时段以及正常脑组织中海马GFAP的表达情况。结果脑缺血再灌注5d内,随着缺血时间的延长,GFAP在海马区星形胶质细胞中的阳性表达逐渐增多,缺血24h后即有表达,缺血再灌注72h后增多,5d后表达最高;经过环磷酰胺处理后,海马区GFAP表达减少(P<0.05)。正常组及假手术组未见GFAP表达。结论大鼠脑缺血再灌注后5d内GFAP在海马中的表达与缺血时间呈明显的相关性,随着缺血时间延长而增加。用环磷酰胺处理后GFAP表达明显减少,提示环磷酰胺对缺血诱导的星形胶质细胞活化具有抑制作用,可能对脑缺血再灌注损伤的恢复起重要作用。
Objective To observe the effect of cyclophosphamide on the expression of collagen fibrillary acidic protein (GFAP) in the hippocampus after cerebral ischemia / reperfusion in rats. Methods The normal group, sham operation group, ischemia / reperfusion group (I / R) and cyclophosphamide group (T) were established. The model of focal cerebral ischemia in SD rats was established by modified Longa thread method. The rabbits in the treatment group were intraperitoneally injected with cyclophosphamide at 1 hour after ischemia. The rats in the treatment group and the I / R group were reperfused at 2 hours after ischemia, and each group was divided into three time periods (24h, 72h and 5d). Immunohistochemistry was used to detect the expression of GFAP in hippocampus in different time points of brain tissue and normal brain tissue. Results Within 5 days after cerebral ischemia / reperfusion, the expression of GFAP in astrocytes of hippocampus gradually increased with the extension of ischemic time. The expression of GFAP increased after 24 hours of ischemia, increased at 72 hours after ischemia-reperfusion, The expression of GFAP in hippocampus decreased after cyclophosphamide treatment (P <0.05). No normal group and sham operation group GFAP expression. Conclusion The expression of GFAP in the hippocampus in 5 d after cerebral ischemia-reperfusion has a significant correlation with ischemic time, which increases with the prolongation of ischemia. Cyclophosphamide treated GFAP expression was significantly reduced, suggesting that cyclophosphamide on ischemia-induced activation of astrocytes have an inhibitory effect may be on the recovery of cerebral ischemia-reperfusion injury play an important role.