应用正交函数分光光度法在干扰组分存在的情况下测定小建中合剂中芍药甙的含量。用电子计算机筛选出测定条件:在217-247nm波长范围,间隔5nm,求7点二次正交多项式系数,可消除制剂中干扰组分对芍药甙含量测定的影响。其平均回收率为100.04％,变异系数CV=0.34％。本法简便、快速、准确。 The content of paeoniflorin in Xiaojianzhong mixture was determined by orthogonal function spectrophotometry in the presence of interference components. The measurement conditions were selected using an electronic computer: In the wavelength range of 217-247 nm, with a spacing of 5 nm, a 7-point quadratic polynomial coefficient was obtained, and the influence of interference components on the determination of paeoniflorin in the preparation was eliminated. The average recovery was 100.04% and the coefficient of variation was CV = 0.34%. This method is simple, rapid, and accurate.