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细胞涂片免疫组织化学染色法，具有取材方便，易行，病人痛苦小，而用于临床。但常因内源性血浆蛋白以及红细胞干扰。使背景出现非特异性染色，不利于观察阳性结果〔１〕。作者采用淋巴细胞分离液处理标本后进行涂片，免疫组化染色可克服上述缺点并能取得比较满意的阳性结果，?
Cell smear immunohistochemical staining method, with access to convenient, easy, patient pain is small, but for clinical use. But often due to endogenous plasma proteins and red blood cell interference. Non-specific background staining, is not conducive to the observation of positive results 〔1〕. The authors used lymphocyte separation liquid samples after smear, immunohistochemical staining can overcome the above shortcomings and can get more satisfactory positive results?