论文部分内容阅读
目的 探讨湖北地区汉族健康人群白细胞介素-1受体拮抗剂基因(IL-1RN)第2号外显子T+8006C位点多态性的分布。方法 采用聚合酶链反应和限制片段长度多态性(PCR—RFLP)方法检测了251名湖北地区汉族健康人群IL-1RN(+8006)位点多态性,并结合文献进行不同种族间的比较分析。结果 湖北地区汉族健康人群基因型以TT型最为常见,其次为TC型,CC型较为罕见,其频率依次为82.9％、16.7％、0.4％;其等位基因以T型最为常见,其次为C型。与美国、德国、日本等国家人群相比,该位点多态性均存在极显著性差异(P<0.005)。结论 湖北地区汉族人群IL-1RN第2号外显子+8006位点存在T/C多态性,其在不同种族间的分布可能存在显著性差异。
Objective To investigate the distribution of T + 8006C polymorphism in exon 2 of interleukin-1 receptor antagonist gene (IL-1RN) in healthy Han nationality in Hubei province. Methods Polymorphisms of IL-1RN (+8006) locus in 251 Han healthy population in Hubei Province were detected by polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) and compared among different races analysis. Results Genotype TT was the most common genotype in Han nationality in Hubei province, followed by TC genotype. CC genotype was rare, with frequencies of 82.9%, 16.7% and 0.4%, respectively. The genotypes of T allele were the most common, followed by C type. Compared with the United States, Germany, Japan and other countries, there were significant differences (P <0.005). Conclusion There are T / C polymorphisms in exon 2 +8006 of IL-1RN in Han nationality in Hubei Province. There may be significant differences in distribution among different races.