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影响外源基因在大肠杆菌中表达的一个因素是翻译起始区(TIR)的二级结构。降低TIR二级结构的稳定性,可以直接提高翻译起始效率,或者间接增高mRNA的稳定性,克服转录极性效应,从而提高外源基因在大肠杆菌中的表达。
One factor that affects the expression of a foreign gene in E. coli is the secondary structure of the translational initiation region (TIR). Decreasing the stability of the TIR secondary structure can directly improve the efficiency of translation initiation or indirectly increase the stability of the mRNA and overcome the transcriptional polarity effect so as to improve the expression of the foreign gene in E. coli.