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２０～３０ｍ ｍ ｏｌ／ Ｌ 半胱氨酸在 ２ｍ ｉｎ 内可与 φ＝ ０．０３ 甲醛完全反应而被掩蔽。利用谷胱甘肽与 ５，５′二硫代双（２硝基苯甲酸）（ Ｄ Ｔ Ｎ Ｂ）的颜色反应，测定产物在波长 ４１２ｎｍ 的 Ｏ Ｄ 值，通过计算或标准曲线可迅速得出谷胱甘肽含量，误差小于 ２％ 。
20 ~ 30mmol / L cysteine in 2min with φ = 0.03 formaldehyde completely masked. Using the color reaction of glutathione with 5,5’-dithiobis (2-nitrobenzoic acid) (D T N B), the O D value of the product at 412 nm was measured and was rapidly calculated or calibrated Derived glutathione content, the error is less than 2%.