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目的:优选冻融-酶解-膜分离复合技术纯化板蓝根多糖的工艺。方法:以板蓝根多糖和剩余蛋白含量为指标,在单因素试验基础上,采用正交试验考察酶解温度、酶解时间、酶用量对板蓝根多糖纯化工艺的影响。结果:最佳纯化工艺为板蓝根残渣水提取液反复冻融3次,过滤,按3.5万U.g-1加入蛋白酶2,酶解温度35℃,酶解时间3 h,酶解后用截留相对分子质量30 kD中空纤维组件超滤。结论:优选的纯化工艺稳定可行,冻融-酶解-膜分离复合技术可用于板蓝根多糖的纯化。
Objective: To optimize the technology of purification of Radix Isatidis by freeze - thaw - enzymolysis - membrane separation and compound technology. Methods: The contents of polysaccharide and residual protein of Radix isatidis were used as indexes. On the basis of single factor experiment, orthogonal experiment was used to study the effects of enzymolysis temperature, enzymolysis time and enzyme dosage on the purification of polysaccharide from Radix isatidis. Results: The optimal purification process was repeated freeze-thawing three times with the aqueous extract of Radix isatidis, filtered, protease 2 was added by 35,000 Ug-1, the enzymolysis temperature was 35 ℃, enzymolysis time was 3 h, 30 kD hollow fiber module ultrafiltration. Conclusion: The optimized purification process is feasible and feasible. The freeze-thaw-enzymolysis-membrane separation and composite technology can be used for the purification of Radix Isatidis.