Objective: To determine the prevalence and economic losses of Fasciola spp. isolates and costs incurred due to liver condemnation from ruminants in Kashan region, center of Iran from 2012 to 2013. Methods: In this cross-sectional study, a total number of 251325 slaughtered sheep, goats and cattle (88939 sheep, 151924 goats and 10462 cattle) were examined via necropsy for fasciolasis in industrial Kashan abattoirs located in center of Iran. The average weight was calculated as 1000 g for sheep, 900 g for goat and 5000 g for cattle in this study region. Based on market value, the average price of a kilo gram of normal liver at Kashan city during the study period was 8 USD. The amount of main nutrients and vitamins elimination in liver contaminated to fascioliasis was determined.Results:Overall 3.28% of the livers were found to be infected. For total number of sheep, goats and cattle slaughtered annually in region study, it was estimated that 7505 livers were infected and total annual economic losses of fascioliasis of studied animals was 41 784 USD (based on market prices in study period). Of this, 23360 USD, 30240 USD and 15400 USD were associated with the fascioliasis of sheep, goats and cattle, respectively. Conclusions: This study indicated that the Fasciola sp. clearly remains prevalent and causes considerable economic losses in study region in sheep, goats and cattle and presumably, other areas and hosts of Iran, providing baseline data for the future monitoring of this potentially important parasitic infection in the country.