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目的了解氢醌(HQ)细胞毒性及DNA损伤作用,探讨HQ遗传毒作用的机制。方法用噻唑蓝(MTT)法检测低浓度HQ对中国仓鼠肺成纤维细胞(V79细胞)作用2 h后,细胞的增殖抑制作用;用单细胞凝胶电泳技术(SCGE)检测不同浓度HQ处理V79细胞2 h后DNA的损伤情况,并观察修复2 h和4 h后彗星图像的变化。结果HQ对V79细胞生长产生明显抑制作用,并呈剂量反应关系。单细胞凝胶电泳结果显示,在设置的5个浓度范围内,彗星细胞拖尾率随着浓度的增加而增加,且有统计学意义(P<0.01);在0.405 mmol/L浓度组,尾长、Olive尾矩、彗星矩和尾/头长4个彗星损伤形态学指标随着修复时间的延长而逐渐降低,其差异有统计学意义(P<0.01)。结论在体外培养条件下,低浓度HQ能明显抑制V79细胞的增殖并呈浓度依赖关系,并能引起DNA损伤,主要是单链断裂,这种损伤部分可自身修复。
Objective To understand the cytotoxicity and DNA damage of hydroquinone (HQ) and to explore the mechanism of genetic toxicity of HQ. Methods MTT assay was used to detect the inhibitory effect of HQ on the proliferation of Chinese hamster lung fibroblasts (V79 cells) for 2 h. Cell viability was measured by single cell gel electrophoresis (SCGE) After 2 h, DNA damage was observed and the changes of comet images were observed 2 h and 4 h after repair. Results HQ significantly inhibited the growth of V79 cells and showed a dose-response relationship. The result of single cell gel electrophoresis showed that the tailing rate of comet cells increased with the increase of concentration within the five concentration ranges set forth (P <0.01). At the concentration of 0.405 mmol / L, tail The length, Olive tail moment, comet moment and tail / head length of four comet injury morphological indicators decreased with the extension of repair time, the difference was statistically significant (P <0.01). Conclusions Under the condition of in vitro culture, HQ at a low concentration can significantly inhibit the proliferation of V79 cells in a concentration-dependent manner and cause DNA damage, mainly single-stranded breaks, which are partially self-repairing.