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背景:闪光视觉诱发电位(flash-visualevokedpotential,F-VEP)是目前儿科临床评估中枢神经系统功能状态的一项新的易行方法。目的:探讨闪光视觉诱发电位(F-VEP),反映新生大鼠缺氧缺血性脑损伤(hypoxic-ischemicbraindamage,HIBD)的敏感性,研究神经生长因子(nervegrowthfactor,NGF)对新生鼠缺氧缺血性脑损伤的保护作用,为新生儿HIBD的早期诊断与干预提供理论依据。设计:完全随机设计,对照实验研究。地点与材料:研究地点为南京医科大学第二附属医院儿科,生后7dSD大鼠来源于南京医科大学实验动物中心,共60只,体质量约14～18g,雌雄不拘,饲养于屏障环境的SPF级实验动物。干预:40只大鼠制成HIBD动物模型后随机分成两组:HIBD模型未治疗组20只,HIBD模型NGF治疗组20只,另20只为正常对照组。主要观察指标:观察正常对照组及HIBD后F-VEP改变;NGF对HIBD模型的新生大鼠体质量增长情况、死亡率、左右脑质量及F-VEP波形影响。结果:HIBD模型NGF治疗组体质量增长(11.9±3.5)g,实验过程中死亡率为5%,患侧(左侧)脑质量(0.59±0.02)与未治疗组相比差异有显著意义(P均<0.01);治疗组左右脑重量差异无显著意义,而未治疗组左右脑质量犤(0.39±0.10)g,(0.57±0.05)g犦差异的显著性意义(P<0.01);HIBD后NGF治疗组与未治疗组即刻F-VEP?
BACKGROUND: The flash-visualevoked potential (F-VEP) is a new and feasible method for pediatric clinical evaluation of functional status of the central nervous system. Objective: To investigate the sensitivity of flash-visual evoked potential (F-VEP) to hypoxia-ischemic brain injury (HIBD) in neonatal rats and the effect of nerve growth factor (NGF) The protective effect of bloody brain injury provides a theoretical basis for the early diagnosis and intervention of neonatal HIBD. Design: Completely randomized design, controlled experimental study. Location and Materials: The study site was Pediatrics of the Second Affiliated Hospital of Nanjing Medical University. After birth, 7-day-old SD rats were derived from Experimental Animal Center of Nanjing Medical University with a total body mass of about 14-18g. The SPF Grade experimental animals. Intervention: Forty rats were randomly divided into two groups: untreated group (HIBD model group) 20, HIBD model group 20 (NGF treatment group) and the other 20 normal control group. MAIN OUTCOME MEASURES: The changes of F-VEP in normal control group and HIBD group were observed. The effects of NGF on the growth of body weight, mortality, left and right brain mass and F-VEP waveform in neonatal rats with HIBD model were observed. RESULTS: The body mass gain (11.9 ± 3.5) g in HIBD model NGF treatment group was 5% in the experiment and 0.59 ± 0.02 in the ipsilateral (left) brain mass. The difference was significant P <0.01). There was no significant difference in the left and right brain weight between the two groups (P <0.01), but there was no significant difference between the left and right brain mass in the untreated group (0.39 ± 0.10 g) and (0.57 ± 0.05) g 犦After NGF treatment group and untreated group immediately F-VEP?