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用NADPH-黄递酶(NADPH-d)组织化学方法结合酪氨酸羟化酶(TH)免疫组化法,对帕金森症模型鼠靶区移植胚腹侧中脑组织后移植区内一氧化氮合酶(NOS)阳性神经元进行观察。结果发现:用6-OHDA损坏一侧黑质纹状体系统后,受损侧黑质纹状体内的TH阳性神经元及神经纤维明显减少,而NOS神经元没有变化。将胚腹侧中脑组织移植到受损侧纹状体后,移植区内TH及NOS阳性神经元存活良好,未见NADPH-d+TH双标记神经元。移植区内NOS神经元有3种形态:一种为大型多极细胞,染色深,可能来自胚黑质致密带外侧;另一种为较小的双极中等程度着色的神经元,可能来自胚脚间核;还有一种NOS神经元,无突起或仅有一条短的突起,细胞酶活性低,仅局限于胞质内,可能是诱导型的NOS神经元。这些结果表明:移植区NOS与TH不共存,黑质纹状体系统的NOS神经元对6-OHDA的毒性不敏感,移植区内不仅有来自胚腹侧中脑NOS神经元,可能还有诱导型的NOS神经元。
Combined with NADPH-diaphorase (NADPH-d) histochemical method combined with tyrosine hydroxylase (TH) immunohistochemistry, the post-transplantation zone of the ventral midbrain tissue of transplanted embryos in the target region of Parkinson’s disease mice was oxidized. Nitrogen synthase (NOS)-positive neurons were observed. The results showed that after the destruction of the nigrostriatal system with 6-OHDA, the TH-positive neurons and nerve fibers in the damaged nigrostriatal striatum were significantly reduced, while the NOS neurons did not change. After transplantation of embryonic ventral midbrain tissue into the injured striatum, TH and NOS-positive neurons in the grafted area survived well. No NADPH-d+TH double-labeled neurons were observed. There are three forms of NOS neurons in the transplanted area: one is a large multipolar cell with deep staining, probably from the outside of the compact substantia nigra; the other is a small, bipolar moderately colored neuron, possibly from the embryo Internucleofoid nuclei; There is also a NOS neuron, no protrusion or only a short protrusion, the cell enzyme activity is low, only limited to the cytoplasm, may be inducible NOS neurons. These results indicate that NOS and TH do not coexist in the transplanted area, and that the NOS neurons in the nigrostriatal system are insensitive to 6-OHDA toxicity. Not only do NOS neurons from the ventral midbrain of the embryo occur in the transplanted area, but there may also be induction. Types of NOS neurons.