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目的观察T细胞缺陷对日本血吸虫感染宿主存活时间,肝脏虫卵肉芽肿形成及日本血吸虫雌虫产卵的影响。方法T细胞缺陷小鼠(裸鼠)和BALB/c小鼠各20只,每只感染日本血吸虫尾蚴(25±1)条,记录感染后存活时间。感染后第42d,从2组存活小鼠中各随机取7只剖杀,取肝组织,计数肝内虫卵;制作肝组织切片,观察虫卵肉芽肿病理学变化,并测量单个虫卵肉芽肿直径。结果20只裸鼠感染日本血吸虫后46d全部死亡。裸鼠肝组织中的雌虫产卵数为(4759.5±2161.3)个/条,BALB/c小鼠为(7323.4±2254.6)个/条,差异有统计学意义(P<0.05)。裸鼠肝脏中单个虫卵肉芽肿直径为(138.4±31.9)μm,与感染组BALB/c小鼠(316.4±68.2)μm比较,差异有统计学意义(P<0.01),且不能形成典型局限性虫卵肉芽肿,肉芽肿内嗜酸性粒细胞明显减少,周围肝组织伴有大面积坏死。结论宿主T细胞对虫卵肉芽肿的形成和维持感染宿主的生存具有重要作用。
Objective To observe the effects of T cell defects on the survival time of host of Schistosoma japonicum infection, the formation of hepatic granuloma and the oviposition of Schistosoma japonicum female. Methods T cell - deficient mice (nude mice) and BALB / c mice (n = 20) were infected with Schistosoma japonicum cercariae (25 ± 1), and the survival time was recorded. On the 42nd day after infection, seven rabbits in each group were randomly divided into seven groups. The liver tissues were counted and the intrahepatic eggs were counted. The liver sections were made and the pathological changes of the granulomas were observed. Swollen diameter. Results All nude mice died after 46 days infected with Schistosoma japonicum. The number of eggs laid in the liver of nude mice was (4759.5 ± 2161.3) / strip, and that of BALB / c mice was (7323.4 ± 2254.6) / strip, the difference was statistically significant (P <0.05). The single worm egg granuloma in the liver of nude mice was (138.4 ± 31.9) μm in diameter, which was significantly different from that of BALB / c mice (316.4 ± 68.2) μm in infection group (P <0.01) Egg granuloma, granulomas within eosinophils decreased significantly, accompanied by a large area of liver necrosis. Conclusion Host T cells play an important role in the formation of ova granulomas and in the maintenance of the survival of infected hosts.