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Aim:To determine whether glycine could downregulate interleukin 1 receptorassociated kinase-4(IRAK-4)expression to interfere with lipopolysaccharides(LPS)signal transduction and blunt transplantative liver ischemia-reperfusioninjury(I/RI).Methods:SD rats were randomly divided into two groups:donoranimals of the glycine group(n=40)were given glycine(1.5 mL;300 mmol/L,iv)1h before harvest,and the control group were treated with 1.5 mL physiologicalsaline(n=40).Orthotropic liver transplantation was then performed according tothe Kamada technique.Ten animals in each group were followed up for 7 d aftersurgery to assess survival.The remaining animals in each group were dividedinto 3 subgroups(n=10)at 1h,2h and 6 h after portal vein reperfusion.Levels ofLPS,serum aspartate aminotransferase(AST),alanine aminotransferase(ALT)and total bilirubin in portal circulation,as well as IRAK-4 and TNF-α expression,NF-κB transcriptional activity and morphological study of liver tissues wereanalyzed.Results:Reperfusion resulted in a significant elevation of LPS concen-trations in each group persisting to the end of our study.However,glycine,whichled to improved survival rate and liver function,significantly alleviated liver pa-renchyma cell damage by downregulating 1RAK-4,TNF-α expression and NF-κBtranscriptional activity compared with the control group.Conclusion:Glycinecan attenuate hepatic I/RI by downregulating IRAK-4 to interfere with LPS signaltransduction.
Aim: To determine whether glycine could downregulate interleukin 1 receptorassociated kinase-4 (IRAK-4) expression to interfere with lipopolysaccharides (LPS) signal transduction and blunt transplantative liver ischemia-reperfusion in jury (I / RI) Two groups: donoranimals of the glycine group (n = 40) were given glycine (1.5 mL; 300 mmol / L, iv) 1 h before harvest, and the control group were treated with 1.5 mL physiologicalsaline was then performed according to tothe Kamada technique. Ten animals in each group were followed up for 7 d aftersurgery to assess survival. The remaining animals in each group were dividedinto 3 subgroups (n = 10) at 1h, 2h and 6h after portal vein reperfusion . Levels of LPS, serum aspartate aminotransferase (AST), alanine aminotransferase (ALT) and total bilirubin in portal circulation, as well as TNF- α expression, NF- κB transcriptional activity and morphological study of liver tissues were observed. Res ults: Reperfusion resulted in a significant elevation of LPS concen- trations in each group persisting to the end of our study. Houever, glycine, whichled to improved survival rate and liver function, significantly alleviated liver pa-renchyma cell damage by downregulating 1 RAK-4 TNF-α expression and NF-κB transcription activity compared with the control group.Conclusion: Glycinecan attenuate hepatic I / RI by downregulating IRAK-4 to interfere with LPS signal transduction.