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目的:比较不同浓度醇沉的瓜蒌薤白提取液的抗氧化性及其药效,以期为瓜蒌薤白提取液的精制选择适当体积分数的乙醇提供理论依据。方法:采用1,1-二苯基-2-苦基肼自由基(DPPH)测定法对5组(未醇沉组与95%,85%,75%,65%乙醇醇沉组)瓜蒌薤白提取液的抗氧化活性进行分析;采用小鼠腹腔注射异丙肾上腺素(Iso)造模,以模型组为对照,测定5组瓜蒌薤白提取液灌胃的小鼠心电图T波变化及血浆中一氧化氮(NO)、超氧化物歧化酶(SOD)含量,考察各组药效。结果:75%醇沉组的抗氧化活性最强,且与未醇沉组相比,有统计学意义(P<0.05);与模型组比较,75%醇沉组在5,10,15,20,30 s,1,2,5,10 min几个时间点能明显抑制T波的改变,且有统计学意义(P<0.05或0.01);与模型组比较,各组均能显著提高小鼠血浆中NO含量,且有统计学意义(P<0.01);与模型组比较,65%、75%和85%醇沉组能显著提高小鼠血浆中SOD含量,且有统计学意义(P<0.05,其中75%醇沉组P<0.01)。结论:75%醇沉组的抗氧化活性及对Iso诱导的小鼠急性心肌缺血模型的保护作用最为显著,可用75%乙醇对瓜蒌薤白提取液进行精制处理。
OBJECTIVE: To compare the antioxidation and pharmacodynamics of white extract of melon grubs with different concentrations of alcohol precipitation, in order to provide a theoretical basis for the selection of the appropriate volume fraction of ethanol for the purification of melons grubs white extract. Methods: Five groups (group without alcohol and group with 95%, 85%, 75% and 65% alcohol alcohol) were treated with 1,1-diphenyl-2-picrylhydrazyl radical The anti-oxidative activity of the extract was analyzed by intraperitoneal injection of isoproterenol (Iso), and the model group was used as the control. The T-wave changes in electrocardiogram (T-wave) and plasma The contents of nitric oxide (NO) and superoxide dismutase (SOD) in each group were measured. Results: Compared with the model group, the 75% alcohol group showed the strongest antioxidant activity and the difference was statistically significant (P <0.05) 20,30 s, 1,2,5,10 min at several time points can significantly inhibit the T wave changes, and have statistical significance (P <0.05 or 0.01); compared with the model group, each group can significantly improve the small Compared with the model group, 65%, 75% and 85% alcohol group significantly increased the content of SOD in the plasma of mice (P <0.01), and there was statistical significance (P <0.01) <0.05, 75% alcohol precipitation P <0.01). CONCLUSION: The anti-oxidative activity of 75% alcohol-treated group and the protective effect of Iso-induced acute myocardial ischemia in mice are the most significant. 75% ethanol can be used to purify the extract from the melon.