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为了阐明芽胞杆菌0-9菌株的生防机制,通过敲除0-9菌株的多糖外运蛋白编码基因pseP构建基因敲除菌株0-9(ΔpseP)和基因敲除菌株的互补菌株0-9(ΔpseP∷pseP),并比较野生型0-9菌株及其衍生菌株形成生物薄膜的能力及其对小麦纹枯病的生防效果。结果显示,pseP基因敲除后,菌株生物薄膜形成能力和对小麦纹枯病的生防作用降低,生防效果较野生型0-9菌株降低43.6%。基因互补后,互补菌株生物薄膜的形成能力和对小麦纹枯病的生防作用得到恢复,生防效果较敲除菌株提高29.2%。研究表明蜡样芽胞杆菌0-9菌株的pseP基因参与了生防菌株0-9生物薄膜的形成和对小麦纹枯病的生物防治。
To clarify the biocontrol mechanism of Bacillus sp. Strain 0-9, knockout strains 0-9 (ΔpseP) were constructed by knocking out the polysaccharide transport protein coding gene pseP from strains 0-9 to complement the strains 0-9 (ΔpseP ::pseP). The biofilm formation ability of wild-type strain 0-9 and its derivative strains and their biocontrol effect on wheat sheath blight were compared. The results showed that the ability of biofilm formation and the biocontrol effect on wheat sheath blight decreased after pseP knockout, and the biocontrol effect was reduced by 43.6% compared with that of wild-type 0-9 strain. After the complementation of genes, biofilm formation of complementary strains and biocontrol effect on wheat sheath blight were restored, and the biocontrol effect was enhanced by 29.2% compared with the knock-out strain. Studies have shown that Bacillus cereus 0-9 strains of pseP gene involved in biocontrol strains 0-9 biofilm formation and biological control of wheat sheath blight.