A new benzophenone glycoside,2,6-dihydroxy-4-O-β-D-glucopyranosylbenzophenone(1),was isolated from the leaves of guajava L.Its structure was elucidated by spectral and chemical methods.1 showed significant activities to secretion of NO in mouse peritoneal macrophages in 10μmol/L. A new benzophenone glycoside, 2,6-dihydroxy-4-O-β-D-glucopyranosylbenzophenone (1), was isolated from the leaves of guajava L. Its structure was elucidated by spectral and chemical methods.1 showed significant activities to secretion of NO in mouse peritoneal macrophages in 10 μmol / L.