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目的:观察益气化瘀解毒法对慢性萎缩性胃炎伴异型增生模型大鼠DNMT1蛋白的影响。方法:将Wistar大鼠60只随机分为造模组50只、空白组10只。造模成功后将造模后剩余的33只大鼠随机分为模型组11只,维酶素组11只,消痞颗粒组11只。空白组、模型组给予生理盐水,维酶素组给予维酶素悬浊液,消痞颗粒组给予消痞颗粒中药制备药液,1次·d-1。连续治疗12周后用Western blot法对各组大鼠DNMT1蛋白进行检测。结果:模型组DNMT1蛋白表达量(0.293±0.036)较空白组(0.133±0.025)显著升高(P<0.01);消痞颗粒组(0.173±0.044)较模型组(0.293±0.036)及维酶素组(0.229±0.032)降低(P<0.05);消痞颗粒组蛋白表达量(0.173±0.044)与空白组(0.133±0.025)比较,差异无统计学意义(P>0.05)。结论:中医益气化瘀解毒法可以逆转胃黏膜异型增生,这种作用可能是通过下调DNMT1而实现的。
Objective: To observe the effect of Yiqi Huayu detoxification method on DNMT1 protein in rats with chronic atrophic gastritis accompanied by dysplasia. Methods: 60 Wistar rats were randomly divided into modeling group 50, blank group 10. After successful modeling, the remaining 33 rats were randomly divided into model group (n = 11), verapamil group (n = 11) and Xiaopi granule group (n = 11). The blank group, the model group was given normal saline, the vitamin group was given vitamin enzyme suspension, Xiaopi particles given Xiaopi granules medicine preparation, 1 times · d-1. After 12 weeks of continuous treatment, the DNMT1 protein in each group was detected by Western blot. Results: Compared with the model group (0.293 ± 0.036), the expression of DNMT1 protein in the model group (0.293 ± 0.036) was significantly higher than that in the blank group (0.133 ± 0.025) (P <0.01) (0.229 ± 0.032) (P <0.05). There was no significant difference in the expression of Xiaopi granule (0.173 ± 0.044) compared with the blank group (0.133 ± 0.025) (P> 0.05). Conclusion: The traditional Chinese medicine Yiqi Huayu detoxification method can reverse gastric dysplasia, this effect may be achieved by down-regulating DNMT1.