【目的】探讨孕期铅暴露对子代大鼠情感行为及学习记忆能力的影响。【方法】通过孕鼠自由饮用0.1%及0.2%醋酸铅溶液的方法建立模型,其雄性子代分别为低剂量铅暴露组(LG)及高剂量铅暴露组(HG),同时设立正常对照组(NG),每组9～12只大鼠。子代大鼠出生后30日龄分别进行旷场试验(open field test,OFT)、避暗试验(pas-sive avoidance test,PAT)以及Morris水迷宫(Morris water maze,MWM)试验,观察各组实验动物情感行为及学习记忆能力的改变。【结果】孕期铅暴露子代大鼠在OFT中穿格得分分值降低(36.48±7.24)(F=11.65,P<0.01),后肢性站立的次数减少(10.61±3.54)(F=9.49,P<0.01),排出粪便粒数增多(19.42±3.46)(F=7.27,P<0.01),提示孕期铅暴露可使子代大鼠情感行为受到损害(P<0.01)。在PAT中,孕期铅暴露子代大鼠犯错次数增加(10.38±1.89)(F=8.69,P<0.01)且记忆潜伏期缩短[(176.41±41.89)s](F=6.48,P<0.01),提示孕期铅暴露可使子代大鼠短期学习记忆能力受损(P<0.01)。Morris水迷宫试验发现孕期铅暴露子代大鼠搜寻站台的逃逸潜伏期明显延长(F=4.61,P<0.05),搜寻策略明显变差(χ2=41.89,P<0.01),站台区域搜寻时间缩短(F=6.32,P<0.01),穿越站台区域次数减少(F=7.13,P<0.01)。提示孕期铅暴露可使子代大鼠空间学习记忆能力受损(P<0.01)。【结论】孕期铅暴露损害子代大鼠的情感行为和学习记忆能力。 【Objective】 To investigate the effects of lead exposure during pregnancy on affective behavior and learning and memory abilities of offspring rats. 【Methods】 Pregnant mice were fed with 0.1% and 0.2% lead acetate solution. The male offspring were exposed to low dose lead exposure (LG) and high dose lead exposure (HG), and normal control group NG), each group of 9 to 12 rats. On the 30th day after birth, the offspring rats were subjected to open field test (OFT), pas-sive avoidance test (PAT) and Morris water maze (MWM) Experimental animals emotional behavior and learning and memory ability changes. 【Results】 The results showed that in offspring of lead exposure during pregnancy, the wearable score of OFT was decreased (36.48 ± 7.24) (F = 11.65, P <0.01) and the number of hind limbs decreased (10.61 ± 3.54) (F = 9.49, (P <0.01). The number of excreted fecal particles increased (19.42 ± 3.46) (F = 7.27, P <0.01), which suggested that lead exposure during pregnancy could impair the affective behavior of offspring rats (P <0.01). In PAT, the number of mistakes of prenatal lead exposure during pregnancy increased (10.38 ± 1.89) (F = 8.69, P <0.01) and memory latency shortened (176.41 ± 41.89) s (F = 6.48, P <0.01) Prompted lead exposure during pregnancy offspring rats can impaired short-term learning and memory ability (P <0.01). Morris water maze test found that the escape latency of search sites of lead exposed offspring during pregnancy was significantly prolonged (F = 4.61, P <0.05), search strategy was significantly worse (χ2 = 41.89, P <0.01) F = 6.32, P <0.01). The number of crossing the platform area decreased (F = 7.13, P <0.01). Prompted lead exposure during pregnancy offspring rats spatial learning and memory impairment (P <0.01). 【Conclusion】 Lead exposure during pregnancy impaired the affective behavior and learning and memory abilities of offspring rats.