本文是对HRP-Protein A间接法检测小麦丛矮病毒的研究报导。试验证明该方法检测寄主植物中的小麦丛矮病毒为阳性反应,健株对照及其它非小麦丛矮病毒均为阴性,对小麦丛矮病毒的检测具有特异性。检测小麦从矮病病株汁液的稀释度达1/1000,在我们的测试条件下,第一抗体浓度1/1000,测试抗体(酶标记A蛋白)的工作浓度1/40为好。 This article is the HRP-Protein A indirect detection of wheat bushy stunt virus research reports. The results showed that this method was positive for detecting wheat cv dwarf virus in host plants. The results showed that the method was negative for both the healthy controls and other non-wheat cv dynas and was specific for the detection of wheat dwarf virus. The dilution of the wheat from the dwarf strain juice was 1/1000. Under our test conditions, the first antibody concentration was 1/1000 and the working concentration of the test antibody (Protein A Protein) was 1/40.