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目的:初步探索灵芝属不同菌种漆酶活性与其生长的相关性,筛选出高产漆酶的菌种。方法:利用PDA固体培养基方式,采用ABTS+法和硫酸-苯酚法分别对漆酶活性和多糖含量进行测定。结果:有柄树舌前期生长较快,漆酶活性第4天达到最大(1 476.64 U/g),为甜灵芝最大值的11.37倍,综合分析后优势显著;信州灵芝多糖含量较低;灵芝S3生长速度平缓,多糖含量居中。结论:灵芝属不同菌种生长速度存在差异,与漆酶活性存在一定的相关性,且有柄树舌可作为漆酶高产的出发菌种。
Objective: To explore the relativity between laccase activity and growth of different strains of Ganoderma lucidum, and to screen out strains of laccase with high yield. Methods: The laccase activity and polysaccharide content were determined by ABTS + method and sulfuric acid - phenol method respectively using PDA solid medium. Results: The early growth of the tree trunks was rapid and the activity of laccase reached the maximum on day 4 (1476.64 U / g), which was 11.37 times of the maximum of sweet ganoderma lucidum. The superiority was obvious after comprehensive analysis; S3 growth rate is gentle, polysaccharide content is medium. CONCLUSION: The growth rate of different strains of Ganoderma lucidum is different, which is related to the laccase activity. And Salvia lanceolata can be used as a starter strain with high yield of laccase.