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通过SSR标记检测了以空心莲子草DNA溶液浸胚处理获得的10个农艺性状稳定遗传的水稻变异系,结果表明,10个水稻变异系均整合了供体空心莲子草DNA的部分片段。在此基础上,以8个水稻变异系及2个对照为试验材料,采用二因素裂区设计,并以主成分分析、逐步回归分析等多种统计方法分析了变异系的耐旱性。结果表明,以综合评价指标与耐旱指数相结合的复合评价体系,可增强水稻耐旱评价的可靠性。导入系H8最耐旱,H6和H7较耐旱,均优于巴西陆稻。本研究结果对水稻的耐旱性评价与耐旱品种选育具有重要意义。
10 SSR markers were obtained by soaking embryos of lotus seed in solution with SSR, and the results showed that all the 10 rice lines were integrated with the DNA of A. philoxeroides. Based on these data, 8 rice mutant lines and 2 control lines were used as experimental materials, and the two-factor split design was adopted. The drought resistance of the variation lines was analyzed by various statistical methods such as principal component analysis and stepwise regression analysis. The results showed that the composite evaluation system which combined comprehensive evaluation index and drought tolerance index could enhance the reliability of drought-resistance evaluation of rice. H8 was the most drought-tolerant and H6 and H7 were more drought-tolerant than that of Brazil. The results of this study are of great importance to evaluate the drought tolerance of rice and breeding drought-tolerant varieties.