【摘 要】
:
Genes homologous to members of the MRP gene family in Caenorhabditis elegans are important in drug resistance.To further explore the molecular mechanism of drug
【机 构】
:
College of Forestry,Northeast Forestry University,Harbin 150040,People’s Republic of China;Heilongji
论文部分内容阅读
Genes homologous to members of the MRP gene family in Caenorhabditis elegans are important in drug resistance.To further explore the molecular mechanism of drug resistance in pine wood nematode(Bursaphelenchus xylophilus),we used bioinformatics approaches to analyze genomic data for B.xylophilus and identified Bx-MRP genes.We predicted the structure and function of the genes and encoded proteins.Using bioinformatics programs to predict and analyze various properties of the predicted proteins,including hydrophobicity,transmembrane regions,phos-phorylation sites,and topologically isomeric structures,of these Bx-MRP genes,we determined that they function in transmembrane transport.From the results of RT-qPCR,the Bx-MRP family members confer significant differential resistance to different drug treatments.After treatment with different concentrations of emamectin benzoate,avermec-tin and matrine,the expression of each gene increased with increasing drug concentrations,indicating that the family members play a positive role in the regulation of multidrug resistance.
其他文献
Tree species dominate many ecosystems throughout the world and their response to climate,in light of global warming,is a matter of primary concern.This review d
市政排水管网是城镇市政管网建设的重要组成部分,随着城镇规模的扩大,排水管网的规模和投资也随之增大,从几个方面介绍城镇排水管网设计过程中的一些重点和难点,以及注意事项
据交通运输部消息,近期,多数港口企业加强了自查自纠,进一步规范拖船、理货、船舶代理等经营行为,促进公平公正竞争,进一步挖掘降本增效的潜力,合理调降了自主定价的港口作业
Understanding the environmental factors that influence tree species composition is essential for suc-cessful management of biodiversity and sustainable use of c
Cyclocarya paliurus is widely distributed in sub-tropical areas of China.Secondary metabolites in the leaves,including flavonoids and triterpenoids,provide prot
To evaluate and select elite Pinus koraiensis par-ent combinations and offspring families, 34 full-sib families were evaluated. Variance analysis of tree height
Relationships between stem growth and climatic and edaphic factors,notably air temperatures and soil mois-ture for different slopes,are not completely understoo
2017年10月23日,菲律宾最大码头运营商菲律宾国际集装箱码头服务公司(International Container Terminal Services Inc,ICTSI)主席兼总裁小恩里克·瑞宗(Enrique K.Razon Jr)
2017年前三季度,珠海港货物吞吐量突破1亿t,全港提前一个季度实现吞吐量破亿t。10月,高栏港区货物吞吐量也突破1亿t。珠海首次实现了双破亿,成功迈向“双亿港口”。珠海市交
Studying diurnal variation in the moisture con-tent of fine forest fuel (FFMC) is key to understanding for-est fire prevention. This study established models fo