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目的:检测生殖细胞核因子(germ cell nuclear factor,GCNF)及Oct-4在宫颈癌HeLa细胞诱导分化前后的表达。方法:用全反式维甲酸(all-trans-retinoicacid,ATRA)诱导HeLa细胞分化,HE染色观察诱导前后细胞的形态变化;通过免疫组化和半定量的RT-PCR方法,检测GCNF及Oct-4在HeLa细胞诱导分化前后的表达。应用SPSS 13.0软件进行统计学处理。结果:随着诱导时间的延长HeLa细胞体积变小,圆形细胞逐渐增多,细胞核开始固缩;GCNF在诱导前后的HeLa细胞中均有表达,随着诱导时间延长其表达逐渐增强但3d后表达强度开始下降,F=14.67,P<0.001。3d组和1d组、2d组和5d组比较差异无统计学意义;但与对照组比较,差异有统计学意义,P<0.05;Oct-4在诱导前后的细胞中均不表达。结论:GCNF参与了肿瘤细胞的体外分化;在体外培养的宫颈癌细胞株中,GCNF基因可能关闭了Oct-4的激素反应元件。
Objective: To detect the expression of germinal cell nuclear factor (GCNF) and Oct-4 in cervical cancer HeLa cells before and after differentiation. Methods: The HeLa cells were induced by all-trans-retinoic acid (ATRA) and the morphological changes of HeLa cells were observed by HE staining. The expressions of GCNF and Oct-1 were detected by immunohistochemistry and semi-quantitative RT-PCR. 4 in HeLa cells induced differentiation before and after the expression. Application SPSS 13.0 software for statistical analysis. Results: The volume of HeLa cells became smaller, the number of round cells increased and the nuclei began to shrink. The expression of GCNF was increased in HeLa cells before and after induction, and gradually increased after induction The intensity began to decline, F = 14.67, P <0.001.3d group and 1d group, 2d group and 5d group, the difference was not statistically significant, but compared with the control group, the difference was statistically significant, P <0.05; Before and after induction of cells were not expressed. CONCLUSION: GCNF is involved in the differentiation of tumor cells in vitro. GCNF gene may shut down the hormone-responsive elements of Oct-4 in cultured cervical cancer cell lines.