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[目的]探讨调肝理脾方对实验性酒精性肝纤维化大鼠的抑制作用及其机制。[方法]Wistar雄性大鼠以“白酒-吡唑-玉米油”混合液灌胃16周制备酒精性肝纤维化大鼠模型,给药后检测肝功能、肝组织病理学变化、肝组织中转化生长因子-β1(TGF-β1)蛋白表达。[结果]酒精性肝纤维化模型大鼠肝功能显著异常,肝纤维化明显;经4周治疗,与模型组比较,中药组及西药组血清中丙氨酸氨基转移酶、天冬氨酸转氨酶、透明质酸水平均有显著下降(P<0.01),且中药组能显著下调大鼠肝组织中TGF-β1蛋白。[结论]调肝理脾方能够有效阻止和逆转酒精性肝纤维化的进程。
[Objective] To investigate the inhibitory effect of Tiaogan Lien Pian Fang on experimental alcoholic liver fibrosis in rats and its mechanism. [Methods] Alcohol-induced hepatic fibrosis rat model was established by intragastric administration of “liquor-pyrazole-corn oil” mixture for 16 weeks in male Wistar rats. After administration, liver function, pathological changes of liver tissue, Transforming growth factor-β1 (TGF-β1) protein expression. [Results] The liver function of alcoholic liver fibrosis model rats were significantly abnormal liver fibrosis significantly; after 4 weeks of treatment, compared with the model group, the Chinese medicine group and western medicine group serum alanine aminotransferase, aspartate aminotransferase , Hyaluronic acid levels were significantly decreased (P <0.01), and the Chinese medicine group can significantly reduce the rat liver TGF-β1 protein. [Conclusion] Tiaogan LIP can effectively prevent and reverse the progression of alcoholic liver fibrosis.