现在,根治慢性骨髓性白血病(CML)仅有同种骨髓移植。但是,移植后经过一定时期仍可再发。再加上为预防骨髓移植后的宿主反应,采用消除T细胞,复发率更高。临床上可一过性出现费城染色体T(Ph′)。所以,骨髓移植能否根绝白血病细胞仍是问题。过去,对残存白血病细胞的检出,仅依赖于细胞形态、染色体像、Ph′等。这些方法不能检出1%以下的白血病细胞。近年,对Ph′易位形成时产生的特异bcr/ab1混合基因,采用聚合酶链反应(PCR),通过人为地增幅达到极高的敏感度,使检出CML中 Currently, there is only one kind of allogeneic bone marrow transplant for chronic myelogenous leukemia (CML). However, after a certain period of transplantation can still be repeated. Coupled with the prevention of host reaction after bone marrow transplantation, the elimination of T cells, the recurrence rate is higher. Philadelphia chromosome T (Ph ’) can occur transiently. Therefore, bone marrow transplantation can eradicate leukemia cells is still a problem. In the past, the detection of residual leukemia cells only depends on the cell morphology, chromosome image, Ph ’and so on. These methods can not detect less than 1% of leukemia cells. In recent years, specific bcr / ab1 mixed genes generated during the formation of Ph ’translocations have been artificially amplified to achieve extremely high sensitivity using polymerase chain reaction (PCR) to detect CML