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目的: 探讨不同胎龄人胎胰腺GnRH-IR细胞的变化。方法:用免疫组化方法对33例第13周至32周人胎胰腺GnRH-IR细胞显示定位后,用体视学方法对其进行形态学测量分析。结果:(1)GnRH-IR细胞分布于胰岛及外分泌部的腺泡、导管上皮细胞间。(2)人胎胰腺GnRH-IR细胞的数量和大小均随胎龄而增大。胰头和胰尾内、外分泌部GnRH-IR细胞的数密度(Nv)、体密度(Vv)、平均截面面积(A )和平均周长(B)均随胚胎发育而逐渐增大。(3)胰头GnRH-IR细胞的分布密度比胰尾的小。胰头内分泌部和外分泌部GnRH-IR细胞的Vv、Nv比胰尾的小(分别P<0.05和P<0.01=。(4)内分泌部GnRH-IR细胞比外分泌部的大而且密集。相同胎龄组同一部位的内分泌部GnRH-IR细胞的Vv、Nv、A、B参数比外分泌部的要大(分别P<0.05和P<0.01)。结论: 胰腺GnRH-IR细胞存在于人胎胰腺导管、腺泡和胰岛,随胎龄而增多增大,胰头部GnRH-IR细胞比胰尾部少,内分泌部的GnRH-IR细胞比外分泌部更多更大。
Objective: To investigate the changes of GnRH-IR cells in human fetal pancreas of different gestational ages. Methods: 33 cases of GnRH-IR cells of human fetal pancreas from the 13th week to the 32nd week were located by immunohistochemistry. Morphometric analysis was performed by stereological methods. Results: (1) GnRH-IR cells distributed in the islets and exocrine acinus, between the ductal epithelial cells. (2) The number and size of GnRH-IR cells in human fetal pancreas increased with gestational age. Number density (Nv), body density (Vv), average cross-sectional area (A) and average circumference (B) of GnRH-IR cells in pancreatic and pancreatic endocrine and exocrine increments gradually increased with embryonic development. (3) The distribution density of pancreatic GnRH-IR cells is smaller than that of pancreatic tail. The Vv and Nv of GnRH-IR cells in pancreatic endocrine and exocrine GnRH-IR cells were smaller than those in pancreatic tail (P <0.05 and P <0.01, respectively). (4) Endocrine GnRH-IR cells were larger and more dense than the exocrine part. The GvR, Nv, A and B parameters of GnRH-IR cells in the endocrine part of the same age group were higher than those in the exocrine part (P <0.05 and P <0.01 respectively) .Conclusion: GnRH-IR cells in pancreas exist in human fetal pancreatic duct , Acinar and islets, with the increase of gestational age increased, pancreatic head GnRH-IR cells less than the tail of the pancreas, endocrine GnRH-IR cells more than the large exocrine.