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将ＰＰＰＡ２Ｐ５Ａ２Ｐ５Ａ（２－５Ｐ３Ａ３）与甲基牛血清白蛋白偶联后免疫Ｂａｌｂ／ｃ小鼠，用杂交瘤技术获得了抗２－５Ｐ３Ａ３单克隆抗体。以〔３Ｈ〕２－５Ｐ３Ａ３作为探针，采用放射免疫沉淀法测定单克隆抗体效价，其单抗经１∶１０００稀释后仍能结合５０％放射性探针，而且有较强特异性，亚型属ＩｇＧ２ａ。
Balb / c mice were immunized with PPPA2P5A2P5A (2-5P3A3) conjugated with methyl bovine serum albumin, and monoclonal antibodies against 2-5P3A3 were obtained by hybridoma technique antibody. Using [3H] 2-5P3A3 as a probe, the titer of monoclonal antibody was determined by radioimmunoprecipitation. The monoclonal antibody could still bind to 50% radioactive probe after 1: 1000 dilution, and had strong specificity , Subtype is IgG2a.