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目的分析A群轮状病毒(RV)引起婴幼儿腹泻的感染情况。方法利用胶体金法A群轮状病毒试剂对本院2012年1月-2012年12月间,15 132例年龄<6岁的腹泻患儿粪便轮状病毒抗原进行检测;对11月份进行了流动患儿和本地患儿的对比分析;对流动患儿和本地患儿的阳性标本随机各抽取10%,共126例样本,采用逆转录PCR(RTPCR)法进行轮状病毒血清G、P基因分型。结果 15 132例样本中阳性3 067例,阳性率20.27%;其中年龄≤6个月的患儿阳性率9.65%(435/4 510);年龄6个月~2岁的患儿阳性率26.55%(2 377/8 953);年龄2岁~6岁的患儿阳性率15.28%(255/1 669);阳性率以10月、11月、12月、1月较高,其中11月最高,为53.71%;11月份流动患儿和本地患儿的对比分析,流动患儿阳性率58.50%(430/735),以G3P[8]为主;本地患儿阳性率51.52%(829/1 609),以G3P[8]为主。结论轮状病毒感染有其特殊性,在年龄上以6个月~2岁患儿感染率最高;还有明显的季节性,以秋冬季感染较高;流动患儿感染率高于本地患儿,且有相同的基因表现型。
Objective To analyze the infection caused by Rotavirus A (RV) in infants with diarrhea. Methods A total of 15 132 cases of diarrhea stool rotavirus antigens from children aged <6 years were collected from January 2012 to December 2012 in our hospital using colloidal gold method A group rotavirus reagents. In November, A total of 126 samples were collected from positive samples of migrating children and local children respectively. RTPCR was used to detect the levels of G, P gene in rotavirus type. Results A total of 3 067 positive samples were obtained from 15 132 samples, with a positive rate of 20.27%. The positive rate of children younger than 6 months was 9.65% (435/4 510). The positive rate of children aged 6 months to 2 years was 26.55% (2 377/8 953). The positive rate of children aged 2 years to 6 years old was 15.28% (255/1 669). The positive rate was higher in October, November, December and January, with the highest in November, (53.71%). In November, the positive rate of migrating children was 58.50% (430/735) compared with that of local children, with G3P [8] as the dominant factor. The positive rate of local children was 51.52% (829/1 609) ), Mainly to G3P [8]. Conclusions Rotavirus infection has its own particularity. The infection rate of children aged 6 months to 2 years is the highest, and the seasonal infection rate is higher in autumn and winter. The infection rate of migrant children is higher than that of local children , And have the same gene phenotype.