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利用PCR－SSCP和DNA测序技术对淮南麻黄鸡CRBP2基因第3外显子的多态性进行了检测，并分析其与蛋品质的相关性。结果表明：在淮南麻黄鸡样本群中CRBP2基因第3外显子存在C／T的突变位点，共检测到3种基因型（ CC、CT和TT），C和T等位基因的频率分别为0?45和0?55，多态信息含量和杂合度分别为0?37和0?47，表明该位点处于中度多态。χ2适合性检验表明淮南麻黄鸡样本群在该位点偏离Hardy?Wein?berg平衡状态（ P＜0?01）。淮南麻黄鸡CRBP2基因第3外显子各基因型间开产日龄、蛋形指数、蛋壳厚度、蛋壳强度、蛋黄色泽和蛋黄比率差异不显著（ P＞0?05），CC基因型的开产蛋重和30周龄蛋重分别比TT基因型高1?66％和2?60％（ P＜0?05），TT基因型的哈氏单位显著高于CC和CT基因型（ P＜0?05）。“,”The polymorphism of exon 3 of CRBP2 gene in Huainan partridge chicken and its associa?tion with egg quality were investigated by PCR-SSCP and DNA sequencing technologies. The results showed that a mutation ( C/T) was found in the exon 3 of CRBP2 gene, three genotypes ( CC, CT and TT) were detected in Huainan partridge chicken, and the frequencies of allele C and T, poly?morphism information content and heterozygosity were 0?45, 0?55, 0?37 and 0?47, respectively, which revealed a moderate polymorphism in this site. Statistical results of χ2 test indicated that this site did not fit Hardy?Weinberg equilibrium ( P0?05 ) , egg weight at first laying and egg weight at 30th week with genotype CC were 1?66% and 2?60% higher than those with genotype TT ( P<0?05) , respectively. The value of Haugh unit with genotype TT was higher than that with genotype CC and CT ( P<0?05) .