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在拟南芥中,NPR1是系统获得抗性SA信号传导途径中的一个重要的调节因子,在水稻中已克隆到与之同源的OsNPR1基因。构建OsNPR1基因水稻过量表达载体,并将其转化粳稻TP309得到转基因植株;通过自交纯合,得到17个纯合株系;对T3、T4代纯合株系进行PCR鉴定,证实转基因纯合株系中外源OsNPR1基因具有遗传稳定性;检测了T1、T2代转基因株系和T3代转基因纯合株系对水稻白叶枯病病原细菌Xanthomonas oryzaepv.oryzae的抗病性,结果表明,在T1、T2代中70%以上的株系对水稻白叶枯病的抗性显著提高,T3代中约67%的株系对水稻白叶枯病的抗性显著提高,说明这种抗病性的提高具有遗传稳定性。OsNPR1基因可作为选育水稻抗白叶枯病新种质的一个良好的候选基因。
In Arabidopsis, NPR1 is an important regulator of systemically acquired resistance to the SA signaling pathway in which the OsNPR1 gene has been cloned. The overexpression vector of OsNPR1 gene was constructed and transformed into japonica rice (TP309) to obtain transgenic plants. Seventeen homozygous lines were obtained by selfing crosses and homozygous lines of T3 and T4 were identified by PCR. The transgenic OsNPR1 gene was characterized by genetic stability. The resistance of the transgenic lines T1 and T2 and T3 homozygous transgenic lines to Xanthomonas oryzaepv. Oryzae was tested. The results showed that in the T1, More than 70% of the T2 generation lines had significantly increased resistance to bacterial leaf blight, and about 67% of the T3 lines showed significantly increased resistance to bacterial leaf blight, indicating an increase in this disease resistance With genetic stability. The OsNPR1 gene can be used as a good candidate for selection of a new germplasm of bacterial blight resistance in rice.