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目的了解应用IHA、ELISA检测鼠疫F1抗体的一致性,评价方法的优劣,以便更好地制订鼠疫监测的方法及防治策略。方法对广西鼠疫监测点隆林、西林县的犬血清使用IHA及ELISA两种试验方法,并进行一致性研究,评价IHA与ELISA方法的可靠性。结果IHA检测鼠疫F1抗体阳性率为0,ELISA检测鼠疫F1抗体的总阳性率为4.4%(12/273);IHA与ELISA之间的一致性为95.6%,其Kappa值等于0,呈轻度一致。结论间接血凝试验方法特异、操作简便是传统的鼠疫监测手段之一;酶联免疫吸附试验具有敏感性高、特异性强、操作简便等特点,且与间接血凝试验有轻度一致性,适宜在鼠疫监测中推广应用。
Objective To understand the consistency of the antibody against F1 in the plague by using IHA and ELISA and to evaluate the merits and demerits of the method in order to better formulate the methods of plague prevention and control strategies. Methods IHA and ELISA were used to detect canine serums in Longlin and Xilin counties in Guangxi plague area. The consistency of IHA and ELISA was evaluated to evaluate the reliability of IHA and ELISA. Results The positive rate of F1 antibody in IHA was 0 and the positive rate of F1 antibody in plague was 4.4% (12/273). The consistency between IHA and ELISA was 95.6%. The Kappa value was equal to 0 and mild Consistent. Conclusion The indirect hemagglutination test is specific and easy to operate. It is one of the traditional means of plague surveillance. Enzyme-linked immunosorbent assay has the characteristics of high sensitivity, specificity and easy operation. It has mild consistency with indirect hemagglutination test, It is suitable for popularization and application in plague surveillance.